Fig. 5

Persistence of RNAi gene knockdown of Dg vATPase A in D. gallinae. Quantitative PCR gene expression analysis of Dg vATPase A expression in adult female D. gallinae mites fed goose blood containing lacZ dsRNA (control) or combined Dg vATPase A dsRNA from R1 and R2. In both the control and Dg vATPase A dsRNA feeding experiments total dsRNA was delivered at 100 ng/μl (final concentration, containing equimolar amounts of R1 and R2 dsRNA), and Dg vATPase A expression levels were monitored over a 120-h post-feed time course. Individual data points for biological replicates are shown with mean ± SEM indicated (n = 3–4). Asterisks represent significant difference (P < 0.05) between treatments determined by Student's t-test