Fig. 3a, b

Characteristics of the Hham-qPCR1 with an EGFP IC. a DNA equivalents of 0.1 Hammondia hammondi oocyst reacted positively in the PCR [colours represent different DNA equivalents: 10⁴ oocysts (red); 10³ oocysts (orange); 10² oocysts (yellow), 10 oocysts (green); 1 oocyst (blue); 0.1 oocyst (black)]. b The Hham-qPCR1 was characterized by a PCR efficiency (E) of 103.9%, and linear regression using DNA equivalents of 10⁴–10⁻¹ oocysts yielded a coefficient of determination (R²) of 99.7%. RFUs Relative fluorescence units, Cq threshold cycle (Ct); for other abbreviations, see Fig. 1