Molecular characterization of RNase III protein of Asaia sp. for developing a robust RNAi-based paratransgensis tool to affect the sexual life-cycle of Plasmodium or Anopheles fitness

Table 2 Validation of the three predicted 3D models of Asaia RNase III by MolProbity, VADAR, Verify_3D and ERRAT

Asaia RNase III model	MolProbity score^a	VADAR Free energy of folding	Verify 3D (%)^b	ERRAT (%)^b	Ramachandran plot quality (%)				Overall quality^c
Asaia RNase III model	MolProbity score^a	VADAR Free energy of folding	Verify 3D (%)^b	ERRAT (%)^b	Most favored	Additionally allowed	Generously allowed	Disallowed	Overall quality^c
Swiss-model	1.66	− 383.46	72.01	91.98	89.2	9.6	1.0	0.2	4.5
Phyre2	3.01	− 191.89	67.07	82.35	85.4	11.3	1.9	1.4	3.5
Modeller	1.72	− 391.65	82.13	100	93.9	4.2	1.4	0.5	6.5

^aMolProbity score combines the clash score, rotamer and Ramachandran evaluations into a single score
^b100 is the best, 0 is the worst
^c10 is the best, 0 is the worst

ISSN: 1756-3305