Fig. 1

a–f Peripheral blood gamont stages of Hepatozoon luiperdjie n. sp. in the African leopard Panthera pardus pardus from hapantotype slide (NMBP392). a, b, e, f Mature gamonts within neutrophils, where enlargement of host cell and displacement of host cell nucleus is apparent. c Extracellular gamont. d Immature gamont. e Mature gamont in which small posterior vacuoles (thick arrow) and thin capsule (thin arrow) can be seen. c, f Disintegration of neutrophils by infecting gamonts. g–k Peripheral blood gamont stages of Hepatozoon ingwe n. sp. in the African leopard Panthera pardus pardus from hapantotype slide (NMBP393). g, h, k Mature gamonts within lymphocytes, where lateral compression of host cell is apparent. g Mature gamont in which bright pink granules and thin capsule (thick arrow) can be seen. h Mature gamont with prominent posterior vacuoles (thin arrow). i Extracellular gamont. j Immature gamont. k Co-infection of Hepatozoon luiperdjie n. sp. (on the left) and Hepatozoon ingwe n. sp. in the same leopard. Scale-bar: 10 µm