Efficacy of a novel orally administered combination product containing sarolaner, moxidectin and pyrantel (Simparica Trio™) against induced infestations of five common tick species infesting dogs in the USA

Table 1 Arithmetic mean tick counts and percent efficacy relative to placebo following one oral treatment with Simparica Trio™ for dogs with existing Amblyomma americanum infestations and after subsequent weekly re-infestations

Study	Tick strain	Day^a	Placebo^b		Simparica Trio™^b		% Efficacy	Test statistic
Study	Tick strain	Day^a	Mean	Range	Mean	Range	% Efficacy	Test statistic
1	Bertek Inc.^c	2	19.0	3–32	0	0–0	100	t₍₁₈₎ = 6.24, P < 0.0001
		7	23.1	14–38	0	0–0	100	t₍₁₈₎ = 10.08, P < 0.0001
		14	20.7	13–33	0	0–0	100	t₍₁₈₎ = 9.10, P < 0.0001
		21	23.1	14–37	0	0–0	100	t₍₁₈₎ = 9.12, P < 0.0001
		28	20.9	13–29	0.1	0–1	99.5	t₍₁₈₎ = 10.05, P < 0.0001
		35	29.7	20–44	0.1	0–1	99.7	t₍₁₈₎ = 13.08, P < 0.0001
2	Ecto Services Inc^d	3	17.7	12–27	0.1	0–1	99.4	t_(9.06) = 10.07, P < 0.0001
		8	23.7	12–35	0.1	0–1	99.6	t_(9.06) = 10.10, P < 0.0001
		15	16.1	5–25	0	0–0	100	t₍₉₎ = 9.11, P < 0.0001
		22	13.9	7–23	0	0–0	100	t₍₉₎ = 9.80, P < 0.0001
		29	12.4	9–16	0.2	0–1	98.4	t₍₉₎ = 16.78, P < 0.0001
		36	14.4	4–25	0.1	0–1	99.3	t_(9.05) = 7.32, P < 0.0001

^aFirst infestation on Day -2. Subsequent infestations on Day 5, 12, 19, 26 and 33. Tick counts were conducted at 48 (Study 1) or 72 (Study 2) hours post-treatment and subsequent re-infestation
^bTreatment with placebo or Simparica Trio™ (minimum dose 1.2 mg/kg sarolaner, 24 µg/kg moxidectin and 5 mg/kg pyrantel (as pamoate salt)) occurred on Day 0
^cColony originated from ticks field-collected in Greenbrier, AR, USA, in 2009 and was last supplemented with wild-caught ticks ~ 3 years prior to study start
^dColony originated from ticks field-collected in Stillwater, OK, USA, in 2011 and was last supplemented with field-collected ticks ~ 4 months prior to study start

ISSN: 1756-3305