Efficacy of a novel orally administered combination product containing sarolaner, moxidectin and pyrantel (Simparica Trio™) against induced infestations of five common tick species infesting dogs in the USA

Table 2 Arithmetic mean tick counts and percent efficacy relative to placebo following one oral treatment with Simparica Trio™ for dogs with existing Amblyomma maculatum infestations and after subsequent weekly re-infestations

Study	Tick strain	Day^a	Placebo^b		Simparica Trio™^b		% Efficacy	Test statistic
Study	Tick strain	Day^a	Mean	Range	Mean	Range	% Efficacy	Test statistic
3	Oklahoma State University^c	2	30.0	3–74	0	0–0	100	t₍₉₎ = 4.78, P = 0.0010
		7	33.8	14–50	0.1	0–1	99.7	t₍₉₎ = 8.12, P < 0.0001
		14	30.1	15–44	0.1	0–1	99.7	t_(9.02) = 10.61, P < 0.0001
		21	32.6	12–47	0.2	0–2	99.4	t_(9.05) = 8.58, P < 0.0001
		28	23.6	6–42	0.3	0–2	98.7	t_(9.06) = 6.27, P = 0.0001
		35	23.1	6–45	0.7	0–2	97.0	t₍₉₎ = 6.31, P = 0.0001
4	Texas A&M University^d	2	35.1	11–50	0.3	0–2	99.1	t_(9.05) = 8.51, P < 0.0001
		7	26.3	0–45	0.3	0–2	98.9	t_(9.04) = 5.43, P = 0.0004
		14	33.9	14–54	0	0–0	100	t₍₁₇₎ = 8.14, P < 0.0001
		21	28.9	13–46	0.7	0–4	97.6	t₍₉₎ = 7.24, P < 0.0001
		28	25.0	13–37	2.4	0–15	90.4	t₍₉₎ = 9.14, P < 0.0001
		35	25.7	6–51	6.1	0–18	76.3	t₍₉₎ = 5.23, P = 0.0005

^aFirst infestation on Day -2. Subsequent infestations on Day 5, 12, 19, 26 and 33. Tick counts were conducted at 48 hours post-treatment and weekly re-infestation
^bTreatment with placebo or Simparica Trio™ (minimum dose 1.2 mg/kg sarolaner, 24 µg/kg moxidectin and 5 mg/kg pyrantel (as pamoate salt)) occurred on Day 0
^cColony originated from ticks field-collected in Stillwater, OK, USA, in 1991 and was last supplemented with wild-caught ticks ~ 15 months prior to study start
^dColony originated from ticks field-collected in Refugio Co., TX, USA, in the mid-1980s and was last supplemented with wild-caught ticks ~ 2 years prior to study start

ISSN: 1756-3305