Table 2 Besnoitia spp. detection by means of PCR and histological techniques in tissues from testicles of acutely infected bulls
Bull | Pampiniform plexus | Testicular parenchyma | Scrotal skin | ||||||
|---|---|---|---|---|---|---|---|---|---|
PCR | qPCR (zoites/mg tissue) | Histology (mean cyst diameter, µm) | PCR | qPCR (zoites/mg tissue) | Histology (mean cyst diameter, µm) | PCR | qPCR (zoites/mg tissue) | Histology (mean cyst diameter, µm) | |
1 | POS | 2.0 × 10−5 | – | NEG | nd | – | POS | NEG | 50.0 |
2 | NEG | nd | 97.1 | POS | 3.0 × 10−3 | 20.0 | POS | 3.8 × 10−4 | 90.7 |
3 | POS | 3.0 × 10−5 | – | POS | 2.7 × 10−1 | – | POS | 3.3 × 10−1 | – |
4 | POS | NEG | – | POS | 3.0 × 10−3 | – | POS | 2.2 × 10−1 | – |
5 | POS | NEG | – | POS | 1.7 × 10−1 | – | POS | 2.0 × 10−4 | – |
6 | NEG | nd | – | POS | 3.0 × 10−3 | – | POS | 1.314 | 25.0 |
7 | NEG | nd | – | POS | NEG | – | POS | NEG | – |