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Table 2 Besnoitia spp. detection by means of PCR and histological techniques in tissues from testicles of acutely infected bulls

From: Vascular wall injury and inflammation are key pathogenic mechanisms responsible for early testicular degeneration during acute besnoitiosis in bulls

BullPampiniform plexusTesticular parenchymaScrotal skin
PCRqPCR (zoites/mg tissue)Histology (mean cyst diameter, µm)PCRqPCR (zoites/mg tissue)Histology (mean cyst diameter, µm)PCRqPCR (zoites/mg tissue)Histology (mean cyst diameter, µm)
1POS2.0 × 10−5NEGndPOSNEG50.0
2NEGnd97.1POS3.0 × 10−320.0POS3.8 × 10−490.7
3POS3.0 × 10−5POS2.7 × 10−1POS3.3 × 10−1
4POSNEGPOS3.0 × 10−3POS2.2 × 10−1
5POSNEGPOS1.7 × 10−1POS2.0 × 10−4
6NEGndPOS3.0 × 10−3POS1.31425.0
7NEGndPOSNEGPOSNEG
  1. Abbreviations: POS, detection of Besnoitia spp. DNA; NEG, absence Besnoitia spp. DNA; nd, not determined; –, cysts not found