Fig. 2
From: Functional characterization of acyl-CoA binding protein in Neospora caninum
Identification and cellular localization of NcACBP. a Expression of NcACBP in E. coli (i) and in N. caninum (ii). b The schematic design of NcACBP endogenously tagged with HA at its C-terminal. The HA tag of NcACBP is fused using single homologous recombination strategy. c Identification of NcACBP-3HA. Lane 1: parental strain Nc-1 as the template; Lanes 2, 3, 4: three clones of NcACBP-HA as the template, respectively. d Location of the NcACBP. NcACBP, stained with mouse anti-HA antibody, was distributed in the cytoplasm of intracellular and extracellular parasites. NcSRS2 was used as a marker to indicate the outlines of parasites, and nuclear DNA was stained with Hoechst (blue). Scale-bars: 1 μm