A molecular survey of vector-borne pathogens and haemoplasmas in owned cats across Italy

Parasites & Vectors

Table 2 Primers and target genes used for pathogen detection in cats across Italy

Pathogens	Primer sequence (5′–3′)	Target gene	Amplicon size (bp)	References
Haemoplasmas	^aMycf: AGCAATRCCATGTGAACGATGAA	16S rRNA	127	[31]
Haemoplasmas	^aMycr1: TGGCACATAGTTTGCTGTCACTT	16S rRNA	127	[31]
Haemoplasmas	^bMycE929f: ACGGGGACCTGAACAAGTGGTG	16S rRNA	259	[26]
Haemoplasmas	^bMycE1182r: AGGCATAAGGGGCATGATGACTTG	16S rRNA	259	[26]
Mycoplasma haemofelis/M. haemocanis	^bRNasePF1: CTGCGATGGTCGTAATGTTG	RNaseP	166	[33]
Mycoplasma haemofelis/M. haemocanis	^bRNasePR1: GAGGAGTTTACCGCGTTTCA	RNaseP	166	[33]
Bartonella henselae/B. clarridgeiae	BART-LC-GEN-F: ATGGGTTTTGGTCATCGAGT	Citrate synthase	190	[32]
	BART-LC-HEN-R: AAATCGACATTAGGGTAAAGTTTTT
	BART-LC-CLA-R: CAAGAAGTGGATCATCTTGG
Ehrlichia spp./Anaplasma spp.	EHR16SD: GGTACCYACAGAAGAAGTCC	16S rRNA	345	[29]
Ehrlichia spp./Anaplasma spp.	EHR16SR: TAGCACTCATCGTTTACAGC	16S rRNA	345	[29]
Babesia spp./Hepatozoon spp.	RLBF: GAGGTAGTGACAAGAAATAACAATA	18S rRNA	460	[28]
Babesia spp./Hepatozoon spp.	RLBR: biotin-TCTTCGATCCCCTAACTTTC	18S rRNA	460	[28]
Filarioids	NTF: TGATTGGTGGTTTTGGTAA	cox1	660	[30]
Filarioids	NTR: ATAAGTACGAGTATCAATATC	cox1	660	[30]

^aPrimers used in real-time PCR for haemoplasma detection and differentiation
^bPrimers used in conventional PCR for haemoplasma detection and differentiation

ISSN: 1756-3305