Exploring the relationship between susceptibility to canine leishmaniosis and anti-Phlebotomus perniciosus saliva antibodies in Ibizan hounds and dogs of other breeds in Mallorca, Spain

Table 7 Estimates of the multivariate models of the relationship between the outcome of the three salivary antigen tests (EU and/or result) and clinical parameters

Model	Factor	Estimate	SE	P-value	Missing observations
SGH result	Breed (Ibizan hound)	1.8057	1.6223	0.1424	0
	L. infantum EU^a	1.8612	0.7809	0.0171*	2
	Age (months)	0.0282	0.0139	0.0423*	7
	Age × Breed^b	− 0.0208	0.0201	0.3024
SGH EU^a	Breed (Ibizan hound)	0.2562	0.0824	0.0026**	0
	L. infantum EU^a	0.1738	0.0709	0.0164*	2
	Age (months)	0.0032	0.0010	0.0028**	7
	Age × Breed^b	− 0.0029	0.0017	0.1057
rSP03B ELISA result	Breed (Ibizan hound)	− 0.2759	0.9843	0.7793	0
	L. infantum EU^a	1.4593	0.7317	0.0461*	2
	Age (months)	0.0295	0.0113	0.0093**	7
	Age × Breed^b	− 0.0118	0.0184	0.5221
rSP03B EU^a	Breed (Ibizan hound)	− 0.0018	0.0704	0.9792	0
	L. infantum EU^a	0.1812	0.0605	0.0037**	2
	Age (months)	0.0037	0.0009	0.0001***	7
	Age × Breed^b	− 0.0013	0.0015	0.8660
RT result	Breed (Ibizan hound)	1.0991	0.6163	0.0745	0
RT result	Age (months)	0.0259	0.0086	0.0003**	7

^aAll EU (SGH, rSP03B, and L. infantum) were log₁₀ transformed prior to modeling
^bInteraction between age (months) and breed group
Abbreviations: ELISA, enzyme-linked immunosorbent assay; EU, ELISA units; GLM, general linear model; IFN-ɣ, interferon gamma; IQR, interquartile range; LST, leishmanin skin test; qPCR, quantitative polymerase chain reaction; RT, rapid test; SE, standard error; SGH, salivary gland homogenate
*P < 0.05, **P < 0.01, ***P < 0.001

ISSN: 1756-3305