One-step RT-qPCR assay for ZIKV RNA detection in Aedes aegypti samples: a protocol to study infection and gene expression during ZIKV infection

Table 1 Primers and probes used in the one-step RT-qPCR assay

Primer/probe	Gene product	Sequence (5′-3′)	Nucleotide position	Amplicon size (bp)	Reference
Zika virus
NS5-2362F	NS5	GACTGGGTTCCAACTGGGAG	2362–2381	96	*
NS5-2457R	NS5	CCACACTCTGTTCCACACCA	2438–2457
ZIKV 1086	Env	CCGCTGCCCAACACAAG	1086–1102	76	[15]
ZIKV 1162c	Env	CCACTAACGTTCTTTTGCAGACAT	1139–1162
ZIKV 1107-FAM	Env	AGCCTACCTTGACAAGCAGTCAGACACTCAA	1107–1137
ZIKV 1086-T7¹	–	TAATACGACTCACTATAGGGAGACCGCTGCCCAACACAAG	–	–	*
NS5-2362-T7 F¹	–	TAATACGACTCACTATAGGGAGAGACTGGGTTCCAACTGGGAG	–	–	*
Aedes aegypti
DefA-F	Defensin A	AACTGCCGGAGGAAACCTAT	122–141	116	[62]
DefA-R	Defensin A	AATGCAATGAGCAGCACAAG	218–237
ActF²	Actin	GAACACCCAGTCCTGCTGACA	583–603	65	[31]
ActR²	Actin	TGCGTCATCTTCTCACGGTTAG	626–647
Act-FAM	Actin	AGGCCCCGCTCAACCCGAAG	605–624

ISSN: 1756-3305