Oncomelania hupensis retains its ability to transmit Schistosoma japonicum 13 years after migration from permissive to non-permissive areas

Table 1 Primers used in the quantitative RT-PCR in the present study

Gene	Sequence (5′–3′)	Primer length (mer)	Tm (°C)	GC %	Product length (bp)
PSMD4	CCTCACCAACAATTTCCACATCT	23	55.50	43.50	129
PSMD4	GATCACTTATAGCCTTGCGAACAT	24	55.80	41.70	129
EWB00_005036	ATACAGAAGCAAGTCCTGAA	20	51.30	40.00	149
EWB00_005036	CTCGCCTAATCCATCTTGT	19	53.01	47.37	149
EWB00_010161	CTGTATCATAGTCGTCGTATC	21	53.66	42.86	104
EWB00_010161	GCATCGTTCCTGTTCCTA	18	52.62	50.00	104
EWB00_002418	TTATACGGTGGAAGCGAAT	19	50.85	42.11	114
EWB00_002418	GGTGGGATATTAGGAGTTCAT	21	53.66	42.86	114
EWB00_003584	TGCTATCGGACTCGTGTA	18	49.80	50.00	122
EWB00_003584	GTAGAGACCATGTATGACAGT	21	50.10	42.90	122
EWB00_003582	GCATTGAATACAGCAGGAC	19	49.70	47.40	136
EWB00_003582	TTATAGCAACGGTTCGGTAT	20	50.00	40.00	136
EWB00_008342	CAATCATCCACTTCAAGATACG	22	53.95	40.91	147
EWB00_008342	CCAGCGAAGCAGGAGTAT	18	54.90	55.56	147
EWB00_005617	GTGGTTCGCTACTGTCAT	18	52.62	50.00	145
EWB00_005617	GATACTCACCGCAACTACA	19	53.01	47.37	145
EWB00_005592	CTTATGCGTCGTGGATGA	18	52.62	50.00	102
EWB00_005592	CCAGGATGACCAGAATGAA	19	53.01	47.37	102
EWB00_010449	TCAATGTTGTCCTGAATGTG	20	51.30	40.00	100
EWB00_010449	TTGTCTTGGTGTTCTTGGT	19	50.85	42.11	100
EWB00_011245	CTGTCACACTACAACAAGAAG	21	53.66	42.86	126
EWB00_011245	GTATTTCGTCACTGCCTTTG	20	53.35	45.00	126
EWB00_009712	GTAATGAACCTACTACTGTTGG	22	53.95	40.91	106
EWB00_009712	GTTCGAGCCTCCTGATTG	18	54.90	55.56	106
EWB00_006199	CAGTTGGAGAGCAAGGAG	18	54.90	55.56	107
EWB00_006199	TGATGGACAGGAAGGAATAC	20	53.35	45.00	107
EWB00_000274	GCAACTTCAAGAACCATACA	20	51.30	40.00	120
EWB00_000274	ATTCCACTACGACCATCTC	19	53.01	47.37	120
EWB00_006201	CCTGGTGAATCTGGACTTG	19	55.16	52.63	139
EWB00_006201	CGCCTCTATCTCCTTCTAATC	21	55.61	47.62	139

ISSN: 1756-3305