Fig. 6

Yolk deposition into oocytes and egg laying by Ae. aegypti in response to non-blood proteins and other factors. a Mean yolk length ± SD 48 h after feeding to repletion on non-blood proteins, skim milk, sucrose or vegetable oil in PBS containing ATP. Each protein was fed to females at 200 mg/ml while other components were fed at concentrations as indicated in "Methods". PBS containing ATP served as the negative control. Small case letters indicate treatments significantly differed from one another after a Kruskal-Wallis and post-hoc Dunn’s test (p ≤ 0.05). b Mean yolk length ± SD 48 h or numbers of eggs laid per female after feeding to repletion on BSA, fatty acid depleted BSA or fatty acid-enriched (AlbuMAX II) BSA. For yolk length no significant difference (ns) was detected among treatments after a Kruskal-Wallis test (p > 0.05). For eggs per female, numbers above each treatment indicate the total number of replete females, horizontal bars indicate the mean ± SD, and different small case letters indicate treatments significantly differed from one another after a Kruskal-Wallis and post-hoc Dunn’s test (p ≤ 0.05). c Mean yolk length ± SD 48 h after feeding to repletion on BSA, peptides derived from BSA, other peptide preparations or amino acids that mimic abundance in BSA. Each treatment was fed to females at 200 mg per ml. Small case letters indicate treatments significantly differed from one another after a Kruskal-Wallis and post-hoc Dunn’s test (p ≤ 0.05)