Table 1 Molecular detection of tick-borne pathogens: target genes, primer nucleotide sequences, amplicon size
Species | Target gene | Nucleotide sequence (5′–3′) | Amplicon size (bp) | Reference |
|---|---|---|---|---|
Rickettsia spp. | ompB | GTAACCCGGAAGTAATCGTTTCGTAA (forward) GCTTTATAACCAGCTAAACCACC (reverse) | 511 | [7] |
Borrelia burgdorferi (s.l.) | Flagellin | AGAGCAACTTACAGACGAAATTAAT(forward) CAAGTCTATTTTGGAAAGCACCTAA (reverse) | 482 | [36] |
Anaplasma phagocytophilum | msp2 | CCAGCGTTTAGCAAGATAAGAG (forward) GMCCAGTAACAACATCATAAGC (reverse) | 334 | [22] |