Fig. 6

CYP4H31 RNAi (si-CYP4H31) in DR strain mosquitoes. Microinjection of an siRNA targeting CYP4H31 in female adult mosquitoes reduced their resistance to deltamethrin. a Microinjection of si-CYP4H31 downregulated the expression of CYP4H31 (41.90%) in the DR stain. b Mosquito mortality was assessed after incubation for a 2 h in a CDC bottle containing 7 mg/l deltamethrin. The mortality of the si-CYP4H31 group was higher than that in the acetone control, WT, and control siRNA (NC2) groups. At 120 min, the mortality rate was 74.3% (29/39) in si-CYP4H31-injected mosquitoes, while it was 46.7% (21/45) in the NC2 group and 48.7% (20/41) in the WT group. Data are representative of three technical replicates of three biological replicates and are indicated as the mean ± SE; **P < 0.01. RE relative expression, DR-strain deltamethrin-resistant strain