Fig 5

Mapping the reactivity of bovine r6cys A and r6cys B polyclonal antibodies against 6cys A and 6cys B synthetic peptides. a Schematic representation of the synthetic peptides selected from the 6cys protein A and B. Peptides A2 and A3 (yellow line), but not A1 (green line) selectively reacted with bovine anti-r6cys A and r6cys B antibodies. Peptide B1 (yellow line), but not B2 (green line) react with bovine anti-r6cys A and r6cys B antibodies. b ELISA analysis. The Y axis represents the average of the optical density (OD) values in ELISA at 450 nm. The X axis represents the serum samples tested (samples taken at 84 dpi for both animal groups). Each bar represents reactivity against distinct synthetic peptides and recombinant proteins, as indicated with different color codes shown at the upright of the chart