Infection, dissemination, and transmission efficiencies of Zika virus in Aedes aegypti after serial passage in mosquito or mammalian cell lines or alternating passage in both cell types

Table 1 Non-synonymous mutations identified in the genomes of Zika virus after six passages in cell culture

Virus passage history	Nucleotide position	Codon sequence^a	Amino acid position	Amino acid
ZIKV-I	256	ATT	C 50	Ile
ZIKV-6C		ATT		Ile
ZIKV-6V		ACT		Thr
ZIKV-6A		ACT		Thr
ZIKV-I	869	AAA	prM 132	Lys
ZIKV-6C		AAC		Asn
ZIKV-6V		AAA		Lys
ZIKV-6A		AAA		Lys
ZIKV-I	2925	GAA	NS1 146	Glu
ZIKV-6C		AAA		Lys
ZIKV-6V		GAA		Glu
ZIKV-6A		GAA		Glu
ZIKV-I	3895	GCG	NS2A 117	Ala
ZIKV-6C		GCG		Ala
ZIKV-6V		GTG		Val
ZIKV-6A		GTG		Val

ISSN: 1756-3305