Table 1 Primer sets of the loop-mediated isothermal amplification for detection of Malaria Pan, Plasmodium vivax and P. ovale
Species | Primer | Sequence (5′–3′) |
|---|---|---|
Malaria Pan LAMP assay | F3 | TGTCAACTACCATGTTACGAC |
B3 | AACGGTCCTAAGGTAGCAA | |
FIP (F1c-F2) | TACGGCCCGACGGTAAGATCGTAACCATGCCAACAC | |
BIP (B1c-B2) | AGGAGTCTCACACTAGCGACAAAATTCCTTGTCGGGTAATCTC | |
LPF | CTGAGCACCTTAACTTCCCTAA | |
LPB | TACACCGTTCATGCAGGAC | |
P. vivax | F3 | CTAATTAGCGGTAAGTACGACA |
B3 | AGCCTAGTTCATCTAAGGACA | |
FIP (F1c-F2) | ACCAAACGCATCAGCTATTCGTATGTCGGATTGGATCTGGA | |
BIP (B1c-B2) | TTACTTGGCTTATCGTACCGTTCAGACCTGTTGTTGCCTT | |
LPF | CACCGACACGAAGTATAATTGC | |
LPB | GCTTCTTAGAGGAACGATGTGT | |
P. ovale | F3 | CGAGTTTCTGACCTATCAGC |
| Â | B3 | GCTGGCACCAGACTTG |
| Â | FIP (F1c-F2) | GATGTGGTAGCTATTTCTCAGGCTCCCTAACATGGCTATGACGG |
| Â | BIP (B1c-B2) | GCAGCAGGCGCGTAAATTACAACCATGAAATGGCCTTGT |
| Â | LPF | TCTCCGGAATCGAACTCTAATTC |
| Â | LPB | TCTAAAGAAGAGAGGTAGTGACAAG |