Effects of Zataria multiflora essential oil on the germinative cells of Echinococcus granulosus

Parasites & Vectors

Table 2 Lethal effect of Z. multiflora essential oil (ZMEO) with different concentrations and at different exposure times on the cultured germinative cells of Echinococcus granulosus

Cons (mg/ml)	Lethal effect on the cultured cells (mean ± SD) (%)*
Cons (mg/ml)	Time	ZMEO	Tween 80**	Albendazole (20 mg/ml)	Negative control
0.5	12 h	0.00^a	0.00^a	–	0.00^a
	24 h	0.00^a	0.00^a	–	0.00^a
	48 h	0.00^a	0.00^a	–	0.00^a
1	12 h	0.00^a	0.00^a	–	0.00^a
	24 h	0.00^a	0.00^a	–	0.00^a
	48 h	0.00^a	0.00^a	–	0.00^a
2	12 h	0.00^a	0.00^a	–	0.00^a
	24 h	20.05 ± 1.86^a	0.00^b	–	0.00^b
	48 h	40.32 ± 2.11^a	0.00^b	–	0.00^b
3	3 h	0.00^a	0.00^a	–	0.00^a
	6 h	29.10 ± 3.63^a	0.00^b	–	0.00^b
	12 h	100 ± 0.00^a	0.00^b	–	0.00^b
4	2 h	51.75 ± 4.47^a	0.00^b	0.00^b	0.00^b
4	3 h	100 ± 0.00^a	0.00^b	–	0.00^b
5	30 min	0.00^a	0.00^a	0.00^a	0.00^a
5	60 min	100 ± 0.00^a	0.00^b	0.00^b	0.00^b
6	30 min	0.00^a	0.00^a	0.00^a	0.00^a
6	60 min	100 ± 0.00^a	0.00^b	0.00^b	0.00^b
7	15 min	45.90 ± 3.00^a	0.00^b	0.00^b	0.00^b
7	25 min	100 ± 0.00^a	0.00^b	0.00^b	0.00^b
8	5 min	46.33 ± 3.79^a	0.00^b	0.00^b	0.00^b
8	7 min	100 ± 0.00^a	0.00^b	0.00^b	0.00^b

ZMEO: Zataria multiflora essential oil, Cons: concentrations, h: hour, min: minutes
*Different letters show significant difference in each row
**Tween 80 was used at twice the concentration of ZMEO

ISSN: 1756-3305