Fig. 1a–c

Fasciola gigantica tegumental calcium-binding EF-hand protein (CaBP) 4 (Fg-CaBP4) sequence and phylogenetic analysis. a The predicted protein homology and secondary structures with the best template found in the Phyre2 search tool, and BLAST search against the National Center for Biotechnology Information (NCBI) non-redundant protein sequence highlighting the two major domains, EF-hand and dynein-light chain, in the Fg-CaBP4 protein. b The predicted 3-dimensional model of Fg-CaBP4 shows 55% similarity to Fasciola hepatica CaBP2 protein (Protein Data Bank: p6422). c A neighbor joining phylogenetic tree showing genetic relatedness between Fg-CaBP4 and CaBPs from other helminth parasites in the NCBI database