Fig. 4

Immunolocalization of CaBP4 within adult F. gigantica flukes. The CaBP4 protein was detected in the tegument and inner lining of the oral sucker and ventral sucker (Acetabulum). Red denotes the target protein stained with cyanine dye 3 (Cy3)-conjugated secondary antibody. Blue denotes nuclei stained with 2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI). No red fluorescence was detected in the control panel. Merge Combined DAPI and Cy3, BF bright fluorescence. Scale bars 250 μm