Fig. 2
From: The effects of plant cysteine proteinases on the nematode cuticle
Immunolocalisation of DPY-7 in WT C. elegans (Bristol N2) cuticles incubated with papain or papain + E64 in a 24-well plate. Whole intact worms were incubated with 1 µM papain or papain + E64 without prior reduction in 1% DTT. a A worm incubated with papain + E64 for 30 min. b Disruption of the C. elegans (Bristol N2 WT) cuticle (arrowed red) after 5 min incubation. c Total disappearance of the collagen stripes and loss of immunoreactivity (yellow brackets) after incubation for 30 min. Bar = 25 µm