Table 1 Primer sequences used for the real-time quantittive PCR assays
Target | Genebank number | Primer sequences (5′ to 3′) | Product size (bp) | Primer length (nt) | Cross intron length (nt) | Primer site |
|---|---|---|---|---|---|---|
IL-1β | NM_008361 | F: AGGAGAACCAAGCAACGACA | 241 | 20 | 1545 | 582…601 |
R: CTCTGCTTGTGAGGTGCTGA | 20 | 822…803 | ||||
TNF-α | NM_013693 | F: GACGTGGAACTGGCAGAAGA | 253 | 20 | 696 | 192…211 |
R: GGCTACAGGCTTGTCACTCG | 20 | 446…427 | ||||
IL-6 | NC_000071 | F: TGCCTTCTTGGGACTGATGC | 216 | 20 | 1272 | 279…298 |
R: GCAAGTGCATCATCGTTGTTC | 21 | 1765…1745 | ||||
Actb | NM_007393 | F: GCCATGTACGTAGCCATCCA | 240 | 20 | 455 | 391…410 |
R: ACGCACGATTTCCCTCTCAG | 20 | 630…611 |