Voltage-sensitive sodium channel (Vssc) mutations associated with pyrethroid insecticide resistance in Aedes aegypti (L.) from two districts of Jeddah, Kingdom of Saudi Arabia: baseline information for a Wolbachia release program

Table 1 Voltage-sensitive sodium channel (Vssc) mutations in Aedes aegypti from Al Safa and Al Rawabi districts, Jeddah, 2018, 2019, 2020 (order of mutations is 1016/1534/989, upper genotype is the base configuration: T = wildtype, G or C = mutant; lower genotype is the amino acid configuration)

Genotype		Safa		Safa		Safa		Rawabi		Rawabi		Rawabi
		Aug-18		Oct-19		Feb-20		Aug-18		Oct-19		Feb-20
		n	Freq	n	Freq	n	Freq	n	Freq	n	Freq	n	Freq
GG/GG/CC GG/CC/PP	K	0	0.00	0	0.00	2	0.03	0	0.00	0	0.00	1	0.02
GG/TG/CC GG/FC/PP	I	1	0.10	1	0.02	2	0.03	0	0.00	0	0.00	0	0.00
GG/TT/CC GG/FF/PP	A	1	0.10	20	0.33	15	0.25	4	0.40	17	0.28	11	0.18
TG/GG/TC VG/CC/SP	J	0	0.00	0	0.00	2	0.03	0	0.00	0	0.00	1	0.02
TG/TG/TC VG/FC/SP	C/L	6	0.60	28	0.47	27	0.45	3	0.30	30	0.50	33	0.55
TG/TT/TC VG/FF/SP	D	0	0.00	0	0.00	0	0.00	0	0.00	0	0.00	1	0.02
TT/TG/TT VV/FC/SS	E	0	0.00	0	0.00	0	0.00	1	0.10	0	0.00	0	0.00
TT/GG/TT VV/CC/SS	B	2	0.20	11	0.18	12	0.20	2	0.20	13	0.22	13	0.22
Total		10		60		60		10		60		60

Test for site differences (all dates combined) χ² = 7.27, df = 7, P = 0.401 (0.388–0.413, 99% confidence intervals)

ISSN: 1756-3305