Fig. 1
From: Requirement of Toxoplasma gondii metacaspases for IMC1 maturation, endodyogeny and virulence in mice
Localization of MCA1 and MCA2. a Localization of MCA1 in a Toxoplasma gondii strain with transient MCA1 overexpression (MCA1-OE). The top and bottom panels showed MCA1 tagged with HA was distributed in the cytoplasm of T. gondii, and was localized in the membrane of tachyzoites as punctate aggregates using anti-HA antibody. MCA1 localized at the IMC as the co-localization of MCA1 with IMC1 were found. The scale bar is 5 μm. b Tachyzoites with MCA2 overexpression using GRA1 promoter was confirmed to fail to multiply (shown as the top panel, MCA2-OE), and the growth of parasites which were not overexpressing MCA2 was normal (shown as the bottom panel, control). TgMCA2 coding sequence tagged with HA at the C-terminus driven by the GRA1 promoter was randomly inserted to the genome of the parasites (as shown in the schematic of the experimental design). The scale bar is 5 μm. c Localization of MCA2 in a T. gondii strain with transient MCA2 overexpression under TgTubulin promoter (MCA2-OE under TgTubulin, as shown in the schematic of the experimental design). MCA2 with HA tag controlled by TgTubulin promoter was distributed in cytoplasm and partially co-localized with IMC1. The scale bar is 5 μm