Fig. 1From: Vectra 3D (dinotefuran, pyriproxyfen and permethrin) prevents acquisition of Borrelia burgdorferi sensu stricto by Ixodes ricinus and Ixodes scapularis ticks in an ex vivo feeding modelSummary of the experiments conducted in this study. a Hair collection on a dog using an individual comb. b Preparation of silicone-based membrane. c Chamber 1 filled with 30 ticks. d Dead ticks assessed after 1 h of DPP-treated dog hair contact. e I. ricinus clustering on the artificial membrane 3Â days after tick infestation. f Feeding units made from two plexiglass chambers, chambers 1 and 2, containing ticks and blood, respectively. g Feeding unit illustration: chamber 1 with organza tissue at the base to restrain ticks; chamber 2 containing blood with end covered with sterile parafilm; artificial membrane glued between chambers 1 and 2. h Cluster of semi-engorged adult I. scapularis ticks feeding on the artificial membrane. i, j Partially engorged I. scapularis ticks after 84Â h of feedingBack to article page