Table 1 Primers, probes and their final concentrations in Toxoplasma gondii real-time PCR assay
From: Establishment and validation of a guinea pig model for human congenital toxoplasmosis
Assay | Names of primers and probes | Sequences of primers and probes 5′–3′ | Probe labelling | Final concentration | References |
|---|---|---|---|---|---|
Toxo529REP PCR | TalF | TGG TTG GGA AGC GAC GAG AG | 800 nM | [41] | |
TalR | CAT CAC CAC GAG GAA AGC GTC | 800 nM | [41] | ||
TalP1_FAM | TGT CGT GCC AGC TGC ATT A | 5′-FAM, 3′-BHQ1 | 200 nM | [41] | |
Internal control PCR, IC2 PCR | EGFP1-F | GAC CAC TAC CAG CAG AAC AC | 500 nM | [42] | |
EGFP2-R | GAA CTC CAG CAG GAC CAT G | 500 nM | [42] | ||
EGFP1-Hex | AGC ACC CAG TCC GCC CTG AGC A | 5′-HEX, 3′-BHQ1 | 160 nM | [42] |