Table 2 Number of proteins identified in saliva of female and male ticks by liquid chromatography-tandem mass spectrometry (LC–MS/MS) and sequential windowed acquisition of all theoretical fragment ion spectra–mass spectrometry (SWATH-MS)
Classification | LC–MS/MS | SWATH-MS (male and females) | |
|---|---|---|---|
Male | Female | ||
Antioxidant/detoxification | 15 | 3 | 10 |
Cytoskeletal | 10 | 1 | 9 |
Extracellular matrix | 4 | 2 | 4 |
Glycine rich | 9 | 1 | 8 |
Heme/iron binding | 0 | 2 | 1 |
Immune related/defence | 8 | 2 | 6 |
Lipocalins | 10 | 11 | 11 |
Metabolism, amino acids | 1 | 1 | 1 |
Metabolism, carbohydrates | 13 | 2 | 8 |
Metabolism, energy | 13 | 1 | 12 |
Metabolism, lipids | 10 | 2 | 6 |
Metabolism, nucleic acids | 7 | 3 | 3 |
Nuclear regulation | 0 | 0 | 1 |
Protease | 18 | 12 | 19 |
Protease inhibitor | 15 | 5 | 9 |
Proteasome machinery | 3 | 0 | 2 |
Protein modification | 14 | 1 | 12 |
Protein synthesis | 1 | 0 | 1 |
Regulation | 11 | 2 | 10 |
Signal transduction | 6 | 0 | 3 |
Transporter/ receptors | 7 | 1 | 5 |
Unknown function | 20 | 12 | 24 |
Total | 195 | 64 | 165 |