Table 1 Gene identification and primer sets of Babesia bovis genes of interest used for PCR and quantitative PCR
Gene identificationa | Locus tag | Forward primers (5′-3′) | Reverse primers (5′-3′) | Sizeb |
|---|---|---|---|---|
cdpk4 (FL) | BBOV_IV003210 | CGCTGCTAAAGTGCAACATATCTTT | CGTGTATGCATTTAGACACCTAGTTT | 1707 |
cdpk4 (qPCR) | BBOV_IV003210 | GGCAGTATGTCGGACAAGGT | CGAACGATCCTTTACCCAGA | 193 |
mt (FL) | BBOV_II003780 | ATGACAGAACTTGCCCATGATCTC | GAAACAGTCTGTAACCTGCGTTT | 234 |
mt (qPCR) | BBOV_II003780 | TGACAGAACTTGCCCATGATC | GGGGAAACATCTTCTTCATCTCA | 102 |
ttl (FL) | BBOV_III004540 | ATGTTAAAGTCAGACATACCCATG | TCTTTGAAAAATTGCAAGTGG | 1242 |
ttl (qPCR) | BBOV_III004540 | TACACTGGGAATTGCACGAA | GACGCCGTGGGTACTTTTTA | 205 |
mapk (FL) | BBOV_IV005520 | CTCCATTGTACAAGTGCCCAAAGGAG | CATGGCTTGTATATAATTTTGAGTGG | 2203 |
mapk (qPCR) | BBOV_IV005520 | GCTTACGTAACCCGCCACTA | ATATCAAAGGCACGGCAGAC | 151 |
α-tubulin (FL) | BBOV_III002820 | GCCAACTTCAATCACTTCATTCCG | GATGCTACGATTAAGTAAATGTTTTTC | 1463 |
α-tubulin (qPCR) | BBOV_III002820 | CATGCTTGACAACGAGGCTA | TGCGAGGGTAAGGTACCAAG | 188 |
hap2 (qPCR) | BBOV_III006770 | AAAGCGTCTATGTAATCAA | ACAGTTTTCTTCTCGTCA | 165 |