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Fig. 2 | Parasites & Vectors

Fig. 2

From: Characterization and evaluation of a recombinant multiepitope peptide antigen MAG in the serological diagnosis of Toxoplasma gondii infection in pigs

Fig. 2

Optimization of MAG-ELISA. a Optimal dilution ratio of MAG antigen and pig serum. b Blocking buffer optimization of BSA concentration and incubation time. c Optimization of pig serum dilution buffer. d Optimization of pig serum incubation time. e Optimization of HRP conjugated recombinant Protein A/G dilution ratio and incubation time. f Optimization of TMB substrate incubation time. g Cutoff value of pig anti-Toxoplasma gondii-negative serum (cutoff = mean + 3 SD)

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Parasites & Vectors

ISSN: 1756-3305

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