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Fig. 4 | Parasites & Vectors

Fig. 4

From: Quantitative PCR assay for the detection of Aedes vigilax in mosquito trap collections containing large numbers of morphologically similar species and phylogenetic analysis of specimens collected in Victoria, Australia

Fig. 4

Aedes vigilax cytochrome c oxidase subunit 1, maximum-likelihood phylogenetic tree including sequences from the two capture locations within Victoria, denoted in boldface. All other sequences were obtained from Puslednik et al. [26]. Trap locations include Victoria, East Gippsland (EAS) and Wellington(WEL); South Australia, St Kilda (SK), Port Adelaide (PA), Adelaide (AD) and Mypolonga (MY); New South Wales, Byron Bay (BY), Port Stephens (PS), Sydney (SY), Shellharbour (SH) and Batemans Bay (BA); Queensland, Cairns (CA); Northern Territory, Darwin (DA); Western Australia, Derby (DE), Broome (BR), Goegrup Lake (GL) and Mandurah (MA); New Caledonia, Noumea (NO). Based on a 591-bp region of the COI gene. General time-reversible (GTR) substitution model was used with 1000 bootstrap replicates. Bootstrap proportions (BSP ≥ 70%) are indicated beside nodes. The number of nucleotide substitutions per site is represented by the scale bar. Aedes procax and Ae. theobaldi were used as an outgroup

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