Fig. 2
From: Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach
Horn fly in vitro feeding trial. a Adult flies in cages feeding for 10Â days upon whole blood collected on days 21, 42, or 56 from animals vaccinated with BI-HS009 or buffer plus adjuvant only. (b Egg collection by allowing oviposition onto moistened filter pads. c Fly eggs on filter paper. d Larval feeding cups containing manure. e Pupae recovered by washing manure onto sieves of different sizes. f Pupae (indicated by arrows) recovered by flotation in a container filled with tap water