Fig. 3

Identification of monoclonal parasite Bxj stably expressing eGFP-BSD. a PCR amplification of F1, F2, and F3 fragments. b Western blot analysis of stable expression of eGFP-BSD in Bxj-c1, Bxj-c2, and Bxj-c3. c IFA confirms stable eGFP expression of Bxj. The parasite nucleus was stained with Hoechst 33342