Table 1 Primers designed by Diawara et al. (2013), and the set designed and optimized by our group (bold italics)
STH | Sense primer (5′-3′) | Antisense primer (5′-3′) | Pyrosequencing primer |
|---|---|---|---|
Codon 167 | |||
T. trichiura | GAGTATCCTGACCGAATTATGACA | (Biot)ACGACGTGAACAGTATCAAACAAC | TGACCGAATTATGACAACT |
N. americanus | GTGACTGTCTCCAGGTAATTCG | (Biot)CTATAACGTACCTTTGGCGAGGG | GATAGAATCATGTCCTCGT |
Codon 198–200 | |||
T. trichiura | CGCCTTTTTAGGTTTCAGATACA (Biot)GCCTTTTTAGGTTTCAGATACA | (Biot)GTCTCCGTAAGTTGGTGTTGTTAA GTCTCCGTAAGTTGGTGTTGTTAA | GGTAGAGAACACGGACG CGCTTCATTATCTATGCAG |
N. americanus | TTTCCGACACTGTGGTTGAG | (Biot)GAGTTCGTTACTAGCCAGCTCACC | GAGAATACAGATGAGACCT AGCTTCATTATCAATACAG |