Fig. 1
From: In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis
Thiacloprid against E. multilocularis metacestodes. a Identification of metacestode and germinal cell. Echinococcus multilocularis GAPDH and mice GAPDH were used for the characterization of E. multilocularis metacestodes and germinal cells (without host cell contamination). Lanes 1 and 5: metacestode; lanes 2 and 6: germinal cells; lanes 3 and 7: mouse liver; lanes 4 and 8: negative control. b Pathological observation of metacestode. Obvious germinal layer and laminated layer were observed in the metacestode section after HE staining. Scale bar = 50 μm. c Metacestode sections were stained with PAS. PAS stain presented a strongly PAS-positive basophilic laminated layer. Scale bar = 50 μm. d Different concentrations of thiacloprid or PZQ against E. multilocularis metacestodes. The thiacloprid or PZQ were tested by PGI assay on E. multilocularis metacestodes in a concentration series ranging from 0.6 to 160 μM in triplicates. Treatment with 0.1% Triton X-100 served as a positive control and was set as 100%. The data are presented as the mean ± SD. e Metacestode vesicle morphology after thiacloprid or PZQ treatment. Metacestode vesicles collapsed after 5 μM thiacloprid or PZQ treatment. f SEM of E. multilocularis metacestodes incubated in vitro with thiacloprid or PZQ. GL: germinal layer