Fig. 4

SJMHE1 treatment modulates Th1/Th2/Th17/Treg cell distribution in the MLNs of mice with DSS-induced acute colitis. On day 10, the mice were killed, and the MLNs from each mouse were tested for Th1/Th2/Th17/Treg subsets through flow cytometry. a–d Proportion of Th1/Th2/Th17/Treg subsets in each treatment group: a CD4⁺IFN-γ⁺ Th1 cells, b CD4⁺IL-4⁺ Th2 cells, c CD4⁺IL-17⁺ Th17 cells, d CD4⁺CD25⁺Foxp3⁺ Treg cells. Data are representative of the experiments. e–h Percentages of Th1/Th2/Th17/Treg cells in each treatment group: e CD4⁺IFN-γ⁺ Th1 cells, f CD4⁺IL-4⁺ Th2 cells, g CD4⁺IL-17⁺ Th17 cells, h CD4⁺CD25⁺Foxp3⁺ Treg cells. Results are presented as mean ± SEM of 18 mice from three independent experiments. Asterisks indicate significant difference at: *P < 0.05, **P < 0.01, ***P < 0.001