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Table 3 Percentage of pairwise divergence among five members of Anopheles hyrcanus group mosquitoes in Japan

From: Updated distribution of anopheline mosquitoes in Hokkaido, Japan, and the first evidence of Anopheles belenrae in Japan

  Specimens 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
1 An. belenrae Akan44 and 9 others, Japan                     
2 An. belenrae isolate 3, South Korea 0                    
3 An. sinensis Yokohama08 and 3 others, Japan 13.25 13.25                   
4 An. sinensis isolate 1, South Korea 13.25 13.25 0                  
5 An. sinensis GLVN59, Vietnam 13.57 13.57 0.26 0.26                 
6 An. engarensis Abashiri02 and 8 others, Japan 13.27 13.27 13.88 13.88 14.2                
7 An. engarensis Daisen55, Japan 14.26 14.26 14.86 14.86 15.19 4.63               
8 An. kleini, South Korea 12.68 12.68 12 12 12.31 6.34 8.67              
9 An. sineroides Fukagawa13 and Kushiro343, Japan 24.58 24.58 25.32 25.32 25.71 20.94 23.76 20.98             
10 An. sineroides SINEK02, South Korea 24.58 24.58 25.32 25.32 25.71 20.94 23.76 20.98 0            
11 An. sineroides Hida386, Japan 24.94 24.94 25.69 25.69 26.08 21.29 24.12 21.33 0.26 0.26           
12 An. sineroides Toyama80, Japan 25.35 25.35 26.11 26.11 26.5 21.67 23.8 21.71 0.8 0.8 1.06          
13 An. lesteri Abashiri01 and 4 others, Japan 34.94 34.94 33.37 33.37 33.82 28.39 32.06 28.8 28.86 28.86 29.24 28.86         
14 An. lesteri Abashiri42, Japan 34.94 34.94 33.82 33.82 34.27 27.98 31.63 29.21 28.86 28.86 29.24 28.86 0.26        
15 An. lesteri Kameda35, Japan 34.94 34.94 33.37 33.37 33.82 28.39 32.06 28.8 28.86 28.86 29.24 28.86 0.26 0.53       
16 An. lesteri Kushiro501, Japan 35.35 35.35 33.77 33.77 34.22 28.77 31.67 29.18 29.24 29.24 29.62 29.24 0.26 0.53 0.53      
17 An. lesteri Kameda38, Japan 36.73 36.73 35.49 35.49 35.95 30.36 34.13 30.78 30.5 30.5 30.89 30.5 1.87 2.14 2.14 2.14     
18 An. anthropophagus SMMU-FK1, China 34.94 34.94 33.37 33.37 33.82 28.39 32.06 28.8 28.86 28.86 29.24 28.86 0 0.26 0.26 0.26 1.87    
19 An. lesteri specimen 1, South Korea 36.88 36.88 36.68 36.68 37.15 31.81 34.35 32.23 32.71 32.71 33.11 32.71 2.97 3.24 3.24 2.69 4.35 2.97   
20 An. lesteri specimen 2, South Korea 42.17 42.17 39.48 39.48 39.97 33.69 37.67 35.31 33.54 33.54 33.95 33.54 6.9 7.2 7.2 7.19 8.67 6.9 8.96  
21 An. pullus, China 38.29 38.29 38.18 38.18 37.73 34.3 33.14 33.98 37.06 37.06 37.48 36.6 41.23 41.23 41.23 40.79 42.17 41.23 43.28 49.18
  1. The number of base substitutions per site from between sequences are shown. Analyses were conducted using the Kimura 2-parameter model [25]. This analysis involved 46 nucleotide sequences. All ambiguous positions were removed for each sequence pair (pairwise deletion option). There were a total of 485 positions in the final dataset
  2. Evolutionary analyses were conducted in MEGA X [26]
  3. Specimens collected in this study are labeled with the species name, specimen name, and country listed in Table 1. Specimens found in GenBank are labeled with species name, specimen name, and country
  4. Values denoted in italic and bold indicate ITS2 intraspecific differences