Table 1 Hc-cbp-1 and Hc-cbp-2 recombinant protein expression and qPCR primers
Gene name | Primer sequence Bold and underlined: restriction sites | Recombinant protein expression (E) or qPCR (Q) primers |
|---|---|---|
Hc-CBP-1 | F:GAATTCTACGGAAGTACTTGGGGATTCTA R:GCGGCCGCTAGTCTTCTAGTCACGTCGTT | E |
Hc-CBP-2 | F: GTCGACCAAGCGGATGTGCTCGCTGCTT R: GCGGCCGCCCCTTGAACATGCCCGGCGA | E |
Hc-cbp-1 | F: ACAAGTTCTACGGCAAGGGA; R: GCGGTGTGTTTGTAGACTCC | Q |
Hc-cbp-2 | F: GCGAGGACGCTTACGAATTA; R: GGAGAAGTCCTCGTAAACAGTG | Q |