Practical guide for microscopic identification of infectious gastrointestinal nematode larvae in sheep from Sardinia, Italy, backed by molecular analysis

Table 2 Summary of the relationship between microscopic identification and molecular analysis of the individual gastrointestinal nematode (GIN) larvae examined in the present study

Microscopic identification	Number of samples	Successfully amplified (PCR)	Successfully sequenced	Correctly identified	Molecular identification	Number of samples
Trichostrongylus spp.	30	22	17	100%	Trichostrongylus colubriformis	12
					Trichostrongylus vitrinus	3
					Trichostrongylus axei	2
Teladorsagia circumcincta	30	22	19	52.6%	T. circumcincta	10
					T. colubriformis	2
					T. vitrinus	2
					T. axei	3
					Cooperia curticei	2
Haemonchus contortus	30	22	16	100%	H. contortus	16
Cooperia spp.	30	23	22	90.9%	C. curticei	20
Cooperia spp.	30	23	22		H. contortus	2
Chabertia ovina	30	16	15	60.0%	C. ovina	9
Chabertia ovina	30	16	15		H. contortus	6
Oesophagostomum spp.	23	12	9	0%	C. ovina	9

ISSN: 1756-3305