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Table 1 Overview of the targets and their respective DNA templates used in multiplex qPCR assay screenings

From: A community approach of pathogens and their arthropod vectors (ticks and fleas) in dogs of African Sub-Sahara

Target Canine blood Tick Flea Limit of detection (copies/PCR) References
Babesia rossi X X   5 [24]
Babesia canis X X   5 [24]
Ehrlichia canis X X   5 [25]
Ehrlichia chaffeensis X X   5 [26]
Anaplasma platys X X   16 [27]
Anaplasma phagocytophilum X X   9 [28]
Rickettsia conorii X X   8 [29]
Rickettsia africae X X   8 [29]
Coxiella burnetti X X   8 [30]
Hepatozoon canis X X   5 In-houseb
Dirofilaria immitis X    8 [31]
Trypanosoma spp.a X    5 [32, 33]
Bartonella henselae    X 5 [34]
Mycoplasma haemofelis    X 16 [35]
Babesia felis   X   8 In-houseb
Dipylidium caninum    X 8 [36]
  1. aviz. Trypanosoma vivax, T. congolense, T. evansi and T. brucei
  2. bHydrolysis probe was designed in-house (Clinomics, Bloemfontein, South Africa)