Oviposition of Culicoides insignis (Diptera: Ceratopogonidae) under laboratory conditions with notes on the developmental life history traits of its immature stages

Table 1 Summary of the oviposition experiments conducted on Culicoides insignis

Experiment	Two-choice oviposition preference assay	Trials (replicates, n )	Blood-fed females (n)	Gravid females, n (%)	Ovipositing females, n (%)	P-value
1	DI vs DI	2 (6)	12	10 (83.3)	4 (40.0)	0.3500
2	DI vs habitat mud	3 (6–8)	21	18 (85.7)	18 (100.0)	0.0780^a
3	Habitat mud + cattle manure vs habitat mud	2 (6)	12	5 (41.7)	4 (80.0)	0.4200
4	Habitat mud vs non-habitat mud	2 (5–6)	11	8 (72.7)	8 (100.0)	0.3400
	Total females, n		56	41	34

^aOviposition preference of C. insignis for mud substrates over DI substrates was marginally significant

ISSN: 1756-3305