Fig. 2

MiR-279-3p targets CYP325BB1 via its 3′-UTR. a A region of the CYP325BB1 3′-UTR containing a putative binding site for miR-279-3p. b The free binding energy of CYP325BB1 targeted by miR-279-3p predicted by the RNAhybrid software. c A dual-luciferase reporter assay demonstrated 33.0% decreased luciferase activity, suggesting that miR-279-3p could bind to the 3′-UTR of CYP325BB1. Data are representative of three technical replicates of three biological replicates and are reported as the mean ± SEM. Asterisks indicate significant difference at **P < 0.01