Fig. 4

MiR-279-3p-mimic-supplied DR-strain mosquitoes by oral feeding reduced the intensity of deltamethrin resistance. a Relative expression of miR-279-3p was upregulated by 8.11-fold in the DR-strain mosquitoes at 48 h after oral feeding with the miR-279-3p mimic. b Relative expression of CYP325BB1 was decreased by 57.5% in the DR-strain mosquitoes at 48 h after oral feeding with the miR-279-3p mimic. c Mortality of the miR-279-3p mimic group after incubation in 7 mg/l deltamethrin-coated CDC bottles for 2 h was higher than that in the acetone control, control and NC groups. The mortality rate ranged from 64.9 to 89.2% in the miR-279-3p mimic group at 90–120 min. Data are representative of three technical replicates of three biological replicates and are reported as the mean ± SEM. Statistical values are relative to the NC. Asterisks indicate significant difference at *P < 0.05, **P < 0.01. Abbreviations: RE, Relative expression