Fig. 2

Spatiotemporal expression of Hc-DAF-5 in H. contortus. a Transcriptional levels of Hc-daf-5 in different developmental stages of H. contortus. Transcript abundance is detected and compared among eight developmental stages including eggs (Egg), the first-stage larvae (L1), the second-stage larvae (L2), the third-stage larvae (L3), the fourth-stage female (L4f), the fourth-stage male (L4m), adult female (Af), and adult male (Am) of H. contortus. The relative quantities (compared with Am, Am = 1, the dotted horizontal line) are shown. All gene transcription levels are normalized to that of the β-tubulin gene. Data are presented as mean ± SEM from three independent experiments. Significant difference is indicated by ** (P < 0.01). b SDS-PAGE analyses of recombinant Hc-DAF-5. Lane M: standard protein molecular weight marker. Expression of Hc-DAF-5 is induced with 1 mM IPTG. Lane 1: recombinant Hc-DAF-5 expressed in E. coli before induction. Lane 2: recombinant Hc-DAF-5 expressed after induction. Lane 3: purified recombinant Hc-DAF-5. c Western blot analyses using total H. contortus protein. Lane 1: detection of Hc-DAF-5 by the rabbit antiserum containing anti-rHc-DAF-5 antibody. Lane 2: the same blot re-probed with pro-immune serum (as control). d Localization of Hc-DAF-5 in adult worms. Images in line 1 and line 2 are taken under UV light corresponding to green fluorescent protein (GFP) and DAPI, and merged images are given in line 3. Scale bars: 100 μm. cu (cuticle), ov (ovary), int (intestine), cem (cement gland)