Table 2 Triplex PCR primers and hybridization probes for O. volvulus (Ov), O. ochengi (Oo), and Onchocerca spp. (16S), with final concentrations in the real-time PCR runs
Primers | Sequence and modification | Concentration (nM) |
|---|---|---|
OvOo ND5 forward | GCTATTGGTAGGGGTTTGCAT | 300 |
OvOo ND5 reverse | CCACGATAATCCTGTTGACCA | 300 |
Ov probe | Fam-TAAGAGGTTATTGTTTATGCAGATGG-BHQ1 | 50 |
Oo probe | Hex-TAAGAGATTGTTGTTTATGCAGATAGG-BHQ1 | 50 |
16S rDNA forward | AATTACTCCGGAGTTAACAGG | 500 |
16S rDNA reverse | TCTGTCTCACGACGAACTAAAC | 500 |
16S rDNA probe | Cy5-TACAACATCGATGTAGCGCAGC-BBQ-650 | 75 |