Metabarcoding in two isolated populations of wild roe deer (Capreolus capreolus) reveals variation in gastrointestinal nematode community composition between regions and among age classes

Table 2 Best generalized linear models selected for ASVs and each diversity index (richness, Simpson, Shannon)

Diversity index	Best generalized models selected	Variables	Parameter estimate ± SE	z-val. or t-val	P
Richness	\(\alpha \sim site\)	Intercept	2.14 ± 0.04	60.91	***
Richness	\(\alpha \sim site\)	SiteTF	0.20 ± 0.06	3.10	**
Simpson	\(\alpha \sim site\)	Intercept	1.01 ± 0.05	18.36	***
Simpson	\(\alpha \sim site\)	SiteTF	0.24 ± 0.12	2.06	*
Shannon	\(\alpha \sim age+site+age\times site\)	Intercept	1.60 ± 0.04	41.85	***
		SiteTF	0.09 ± 0.09	1.01	–
		AgeY	– 0.02 ± 0.07	– 0.30	–
		AgeY:siteTF	0.32 ± 0.13	2.41	*

Gaussian families were used for Simpson and Shannon regression and Poisson family for richness. The effect of site (Chizé as reference), age (adult as reference) and sex (females as reference) and the interaction between them are reported when included in the selected models. Parameter estimates with SE are reported with the corresponding z-value (Poisson family) or t-value (Gaussian family) and P-value. Statistical significance is represented by *P < 0.05, **P < 0.01 and ***P < 0.001. TF: Trois Fontaines; Y: young

ISSN: 1756-3305