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Table 3 Data of the 21 small mammals that were shown to contain Hepatozoon DNA by two PCR protocols, one targeting a small fragment (≈540 bp) and the other targeting a large fragment (≈1700 bp) of the Hepatozoon 18S RNA gene

From: Novel genotypes of Hepatozoon spp. in small mammals, Brazil

Mammal individual Code

Areaa

Mammal species

Results of PCR targeting the 18S rRNA gene

Positive samples

Small fragment haplotypeb

Large fragment haplotypec [GenBank accession number]

PR065

A4

Nectomys squamipes

Spleen

1d

 

RP033

A5

Oligoryzomys sp.

Lung

1

 

HF006

A6

Akodon sp.

Lung, liver, spleen

2

 

HF010

A6

Akodon sp.

Liver

2

 

HF015

A6

Akodon sp.

Liver

2

HF015 [OM033663]

HF025

A6

Akodon sp.

Blood

2

 

HF027

A6

Akodon sp.

Lung

2

 

HF005

A6

Akodon sp.

Lung, liver, spleen

2

HF005 [OM033660]

HF009

A6

Euryoryzomys russatus

Lung

2

HF009 [OM033661]

HF014

A6

E. russatus

Lung

2

HF014 [OM033662]

HF021

A6

E. russatus

Lung

2

 

PS003

A7

Gracilinanus microtarsus

Liver

4

 

PS001

A7

Monodelphis domestica

Lung, spleen

4

PS001 [OM033664]

PS063

A7

M. domestica

Lung, liver, spleen

4

 

PS082

A7

M. domestica

Liver, spleen

4

 

PS113

A7

M. domestica

Lung, liver, spleen

4

 

PS130

A7

M. domestica

Lung, spleen

4

 

PS085

A7

Oecomys marmorae

Lung

3

PS085 [OM033665]

PS119

A7

O. marmorae

Lung, spleen

3

 

PS125

A7

O. marmorae

Lung, liver, spleen

3

 

PS022

A7

Thrichomys pachyurus

Lung

3

 
  1. aAreas A4, A5, A6, and A7 are indicated in Fig. 1;
  2. bsee Fig. 2;
  3. csee Fig. 3;
  4. dGenBank accession number for haplotype 1 is OM033659; haplotypes 2, 3, and 4 are within the respective large fragment haplotypes, for which GenBank accession numbers are provided in the last column of the Table