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Table 4 Distribution of the parasitic load detected using H&E staining, IHC and qPCR, and the association with severity of dermal inflammation

From: Comparison of immunohistochemical and qPCR methods from granulomatous dermatitis lesions for detection of leishmania in dogs living in endemic areas: a preliminary study

Severity of dermal inflammation
(N)
Parasite load
H&E staininga IHCa qPCRa
Negative (−) Suspect
(+/–)
Mild (+) Moderate (++) Intense (+++) Negative (−) Suspect
(+/–)
Mild (+) Moderate (++) Intense (+++) Negative (−) Low (+) Moderate (++) Intense (+++)
Mild (+)
(6)
5 0 0 0 1 5 0 0 0 1 2 3 0 1
Moderate (++)
(13)
7 2 1 1 2 7 1 1 3 1 3 4 2 4
Severe (+++)
(11)
5 3 0 2 1 3 0 6 0 2 4 0 3 4
χ2 (p) 5.7 (0.77) 15.64 (0.029)* 7.34 (0.29)
r (p) 0.18 (0.33) 0.26 (0.099) 0.13 (0.41)
  1. Numbers in table are the number of samples, unless indicated otherwise
  2. *Statistical difference
  3. aSee Methods section for a description of the grading system applied