Detection of specific antibodies against Leishmania infantum in canine serum and oral transudate using an in-house ELISA

Table 1 Signalment and geographical distribution of dogs enrolled in the study

Geographical distribution (number of dogs)	Sex (%, number of dogs)		Breed (%, number of dogs)		Most common breeds (%, number of dogs)	Age (%, number of dogs)^a		Age, median (years, min–max)^{a, e}	Clinical status (%, number of dogs)
Geographical distribution (number of dogs)	Female^b	Male^b	Purebred^c	Crossbreed^c	Most common breeds (%, number of dogs)	Young^d	Adult^d	Age, median (years, min–max)^{a, e}	Healthy^f	Sick^f
Asturias (47)	51.1%, 24	48.9%, 23	89.4%, 42	10.6%, 5	English setter (17%, 8) and Mastiff (10.6%, 5)	8.9%, 4	91.1%, 41	5.5%, 0.5–12	100%, 47	0%, 0
Barcelona (110)	46.4%, 51	53.6%, 59	20.9%, 23	79.1%, 87	German Shepherd (4.5%, 5) and Labrador Retriever (3.6%, 4)	25.5%, 24	74.5%, 70	4%, 0.3–12	87.3%, 96	12.7%, 14
Cádiz (54)	44.4%, 24	55.6%, 30	29.6%, 16	70.4%, 38	Spanish sighthound (11.1%, 6)	17.5%, 7	82.5%, 33	3.5%, 0.5–16	100%, 54	0%, 0
Mallorca (94)	68.1%, 64	31.9%, 30	80.9%, 18	19.1%, 76	Ibizan Hound (54.3%, 51), Mallorca Shepherd dog (5.3%, 5) and Andalusian wine-cellar rat-hunting dog (5.3%, 5)	38%, 35	62%, 57	3%, 0.5–14	92.6%, 87	7.4%, 7
Total of provinces of origin (407)	51.4%, 209	48.6%, 198	46.7%, 190	53.3%, 217	Ibizan Hound (12.8%, 52), German Shepherd (3.9%, 16) and Mastiff (3.4%, 14)	22.8%, 79	77.2%, 267	4%, 0.3–16	89.9%, 366	10.1%, 41

max maximum, min minimum
^aAge was not recorded in 2 dogs from Asturias, 16 dogs from Barcelona, 14 dogs from Cádiz, 2 dogs from Mallorca and 27 dogs from other Spanish regions
^bMallorca had a higher rate of female dogs (Chi-square test: X² = 11.7, df = 3, P = 0.008)
^cAsturias and Mallorca had a higher rate of purebred dogs (Chi-square test: X² = 110.9, df = 3, P < 0.001)
^dMallorca had significantly more young dogs than Asturias (Fisher’s exact test: P < 0.0001) and Cádiz (Fisher’s exact test: P = 0.025), while Asturias had significantly more adult dogs than Barcelona (Fisher’s exact test: P = 0.024)
^eDogs from Mallorca were significantly younger than those from Asturias (Mann–Whitney test: U = 2740, n₁ = 45, n₂ = 92, P = 0.002) and Barcelona (Mann–Whitney test: U = 5106, n₁ = 94, n₂ = 92, P = 0.032)
^fBarcelona and Mallorca had some sick dogs (Chi-square test: X² = 13.4, df = 3, P = 0.004) while all dogs in Asturias and Cádiz were apparently healthy

ISSN: 1756-3305