Sensitivity of Haemonchus contortus to anthelmintics using different in vitro screening assays: a comparative study

Table 4 Activity of a novel series of benzimidazole derivatives (at 25 µM) for each Haemonchus contortus assay (incubation time of 72 h for xL3 or adult stage motility test and 7 days for L4 development test)

Compound ID^a	% of motility on xL3s (average ± SEM)	% of L4 development (average ± SEM)	Adult stage motility score (average ± SEM)
1a	93.1 ± 30.1	67.2 ± 4.9	2.2 ± 0.4
1b	131 ± 33.5	103.8 ± 9.6	1.5 ± 0.5^d
1c	144.8 ± 39.5	106.1 ± 9.4	2.0 ± 0.0
1d	177.6 ± 56.1^b	68.2 ± 18.4	0.1 ± 0.1^d
1e	179.6 ± 49.9^b	30.9 ± 15.1^c	0.1 ± 0.1^d
1f	113.8 ± 25.7	101.9 ± 5.4	2.0 ± 0.0
2a	193.1 ± 51.2^b	111.2 ± 9.1	1.2 ± 1.1^d
2b	131.0 ± 34.9	108.9 ± 6.7	2.3 ± 0.5
2c	134.5 ± 33.9	101.4 ± 13.6	1.4 ± 0.5^d
3a	193.3 ± 32.3^b	105.5 ± 7.4	2.2 ± 0.4
3b	156.7 ± 18.1^b	100.0 ± 8.3	2.2 ± 0.4
ABZ ^e	29.4 ± 11.1^b	0.6 ± 1.2^c	1.0 ± 0.3^d

Bold numbers indicate that the compound was considered active
^aThe chemical synthesis and structure of the compounds was previously reported by [24]
^bMotility was significantly different from motility of DMSO control (P < 0.05) (see “Statistical analysis” section for further details)
^cDevelopment was significantly different from development of DMSO control (P < 0.05)) (see “Statistical analysis” section for further details)
^dMotility score of a compound at 72 h time point ≤ 1.5 is considered active[19]
^eABZ was evaluated at a concentration of 20 µM

ISSN: 1756-3305